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Cells were then transfected with either the pcDNA3 vector (Invitrogen, Waltham, MA, USA) alone as a negative control or with GSK3 or DYRK1A cDNA using JetOptimus transfection reagent (Polyplus transfection, Illkirch, France) following the manufacturers instructions

An update

Tumor cells can develop adaptive mechanisms to resist ferroptosis induction, such as upregulating antioxidant systems or altering iron metabolism pathways

Creatine is a natural acid produced by the kidneys and liver and mostly stored in the muscle cells

Katie emphasizes the importance of creating research that's "scientifically informative and interesting" while remaining realistic about dietary supplements' capabilities