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Slides were mounted after rinsing in PBS with mounting medium (Agilent Dako), dried overnight at room temperature and stored at 20 C until imaging

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Several nutrients have protective effects on the ocular surface, such as omega-3 and omega-6 fatty acids for reducing inflammation, vitamin D for dry eye risk reduction and recovery enhancement, vitamin A supporting the corneal epithelium, vitamin B12, and citicoline improving ocular surface health, vitamin C protecting the eye's tear film, and vitamin E for oxidative stress reduction and corneal healing