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To test for changes in neurochemical levels over time, linear mixed-effects models were run using the lmer function in the lme4 package (version 1.1.27.1) 27 , with timepoint (1 or 2) and group (Con-Con, Mig-Mig, Con-Mig) included as fixed effects (including a timepoint by group interaction), and subject included as a random effect

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We also examined gene expression changes induced by C1-27 in HCT116 cells after 4 and 24 h treatment to observe the early and late response to GSTO1 inhibition, respectively (Supplementary Tables 1417 and 2023)

Quick and Comfortable Infusion: The treatment is administered via an IV drip, lasting between 30 to 90 minutes depending on the personalized dosage