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The same group saw similar effects in vitro and in vivo in a cigarette smoke extract (CSE) and LPS model of COPD, along with suppression of pulmonary fibrosis [75]

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FIGURE 8 3.8 Immunohistochemical findings The immunohistochemical investigation demonstrated a significant increase in expression of proapoptotic makers, Bax, and caspase-3, in MTX treated group, which was associated with decreased antiapoptotic marker, Bcl-2, expression when compared to the CTRL group (Figure 9)

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