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Mitochondrial respiratory complex activity measurement The enzymatic activities of complex I (NADH ubiquinone reductase), complex II (succinate ubiquinone reductase), complex III (ubiquinol cytochrome C reductase), and complex IV (cytochrome C oxidase) were measured in macrophages using enzyme activity assay kits (A089-1-A089-4, Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturer's instructions

A clinical trial has shown that acetazolamide effectively increases the mean pulmonary artery flow acceleration time while reducing the pulmonary vascular resistance index in patients with CMS (Leon-Velarde et al., 2010)

To gain a deeper insight into disease mechanisms, analyses were simultaneously conducted on both plasma and pericardial fluid

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