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For immunoprecipitation, anti-FLAG (Sigma-Aldrich, M2, A2220), anti-HA (Sigma-Aldrich, A2095), and anti-OTUD5 (Cell Signaling Technology, 20087, 1:1,000) antibodies were used

The L/P/S cocktail did not show a significant anti-depressant effect that might be the result of the depression-precipitating nature of long-term administration of pregabalin (56) and scopolamine (59)

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Generally, as described by Becker et al