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In addition, night feedings, a snack before bedtime or maize starch supplementation at 2 g per 1 kg body weight are recommended to maintain adequate blood glucose levels during sleep [97,98]

(3) Place the filter paper and gel together in the membrane transfer buffer

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Dissociated cells were suspended in L-15 medium (Liebovitz, L1518, Merck) containing 10% fetal bovine serum, NaHCO 3 , glucose, penicillin/streptomycin (1) and floxuridine (PHR2589, Merck) and plated on coverslips precoated with poly-d-lysine (A-003-E, Merck) and laminin (L2020, Merck)

Polyvinyl-pyrrolidone-coated silver nanoparticles-the colloidal, chemical, and biological consequences of steric stabilization under biorelevant conditions