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The AcGFP-tagged RNF213 was cloned into the pJL-NeoR vector and was co-transfected into RNF213 -knockout cells with pJL-SB vector using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, 11668019)

Inositol plays important roles in cellular signalling, particularly in insulin signal transduction and glucose metabolism