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Impure peptide preparations can introduce experimental confounders, as degradation products or synthesis byproducts may have their own biological activities or interfere with receptor binding assays
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For instance, structural analysis of the anti-PCSK9 mAb 1D05 32 reveals that the light chain N-terminus is close to the binding interface and a peptide fusion at this locus is likely to significantly alter the antibody binding properties
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