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American Psychiatric Association (2022) Diagnostic and statistical manual of mental disorders

And more importantly, is it safe

In healthy individuals, FMO3 rapidly converts TMA to odorless TMAO

This protocol is an extension of the widely accepted PEN-FAST (penicillin allergy reported by patient, five years or less since reaction, anaphylaxis or angioedema, severe cutaneous adverse reaction, and treatment required for reaction) clinical decision tool that has been used to identify patients with reported penicillin allergy who are appropriate for DOC rather than immediate hypersensitivity skin testing, which recent data suggest has poor positive predictive value in low-risk patients

If you added 1mL instead of 2mL, your concentration is 5mg/mL, double what you calculated for