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Risk-of-bias assessment The methodological quality of included RCTs was assessed utilizing the Cochrane Risk of Bias 2 (RoB2) tool [18], while observational studies were evaluated with the Risk Of Bias In Non-randomized Studiesof Interventions (ROBINS-I) [19]

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Pull down assay for GPX4 PC12 cells were treated with DAQ probe (DA-yne probe contains FeCl 3 solution) or DMSO for 6 hours, and then were harvested and lysed with RIPA buffer supplemented with protease inhibitor cocktails and phosphatase inhibitor cocktails

Ipamorelin currently lacks FDA approval and remains a research peptide that has no approved medical uses

doi: 10.3390/ijms241713316 93 HallJRalphECShankerSWangHByrnesLJHorstRet al