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A single colony of each strain was inoculated into 5 mL of LB medium for 68 h with shaking at 37C and 200 rpm

The resulting 2.5% SSA plasma supernatant was collected into 1.5 mL microcentrifuge tubes, flash frozen on dry ice, and stored at 80 o C for future analysis

Two 45 L aquariums, with outer layers covered in white plastic, were used

Aside from being older, other risk factors could lead to NAION

If you or someone in your family has had medullary thyroid carcinoma or Multiple Endocrine Neoplasia syndrome type 2 (MEN 2) or another endocrine neoplasia syndrome type, or other medical conditions, you should not take this medication