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After collecting whole-cell extracts (WCE), the remaining lysate was centrifuged (2000 g , 5 min) to isolate chromatin-enriched pellet (chromatin) and supernatant (SN)

As soon as the catalytic mechanism of GPx was discovered, the design and development of synthetic compounds mimicking GPx-like activity became an active research area [71,72,73]

Moreover, glutathione itself is known to also be produced rhythmically and so the same logic applies to any NRF2 activator when viewed as a therapeutic in this disease context

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