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Cellular assays have enabled detailed examination of second messenger dynamics, such as cyclic AMP generation and calcium mobilization, while animal models have provided insight into systemic signal coordination and feedback regulation along the hypothalamic pituitary axis

After co-incubating for 24 h, cells were harvested by centrifugation and subjected to flow cytometer

Visceral fat produces inflammatory cytokines, contributes to insulin resistance, and correlates strongly with cardiovascular disease risk

Diez-Caballero F, Castilla-Cortazar I, Garcia-Fernandez M, Puche JE, Diaz-Sanchez M, Casares AD, Aliaga-Montilla MA, Rodriguez-Borrajo C, Gonzalez-Baron S: Little effects of insulin-like growth factor-I on testicular atrophy induced by hypoxia

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