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0.22 m) immediately prior to use Aliquot and store reconstituted solutions at 20 C (or lower) to minimise degradation Avoid repeated freezethaw cycles Due to reported low intrinsic stability in vitro, prepare fresh working solutions when possible Specifications Summary Precautions & Notes Experimental behaviour of DSIP is dependent on concentration, exposure duration, and model system Buffer composition, pH, and enzymatic activity may influence peptide stability Due to reported rapid degradation in some in vitro systems, appropriate controls are recommended Literature reports describe heterogeneous and sometimes contradictory findings across models Intended strictly for laboratory research use
Garcia-Contreras M, Thakor A
Med Clin (Barc) 155:256262 Zhang Y, Khoi PN, Cai B, Sah DK, Jung Y-D (2022) Sulforaphane regulates eNOS activation and NO production via Src-mediated PI3K/Akt signaling in human endothelial EA.hy926 cells
As a result , investigators focus on mechanistic pathways and molecular interactions rather than clinical effects
