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The present study also explores the possibility that the oxidized inactive form of e-GST may be present in blood, given that two critical cysteines, Cys47 and Cys101 (the latter corresponding to Cys103 in pig e-GST) are strictly conserved in all mammals tested, and that they form a disulfide both in vivo and in vitro as proved for the human enzyme

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Wipe each rubber stopper with an alcohol pad in one direction