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Normal thyroid function is regulated by the hypothalamic thyrotropin-releasing hormone (TRH) that stimulates the release of the thyroid-stimulating hormone (TSH) from the pituitary gland, which in turn drives synthesis and secretion of the thyroid hormones from the thyroid gland ( th week of gestation, the embryonic thyroid is in its final position in front of the trachea

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The seeding medium consisted of DMEM (Gibco, #31966-021) supplemented with 10% v/v FBS (Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122)