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reported that embryonic glucagon-expressing cells also expressed PC1/3 and that the level of PC1/3 expression decreased with age ( ARX and DNMT1 , are differentially expressed across alpha-cell subpopulations, in which pseudotime analysis suggested that certain subpopulations are more mature than others ( PCSK1 (gene name for PC1/3) ( PCSK1 -expressing alpha-cell subpopulation in response to intra-islet GLP-1R signaling ( TOP2A , MKI67 , CENPF , BIRC5 , and CDK1 ), suggesting that one of these subpopulations, albeit the smaller subpopulation, of alpha-cells may constitute proliferating alpha-cells ( TOPA1 , CENPF , and AURKB ( Further technical advances have enabled researchers to validate single-cell transcriptomes with direct functional correlates in the same cell, resulting in more precise measures of alpha-cell heterogeneity