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Following fixation in 4% paraformaldehyde (PFA), 5 dpf Brn3c:mGFP larvae, which express membrane-bound GFP in hair cells, were rinsed twice with phosphate-buffered saline (PBS) for 10 min each and then once with dH 2 o for 20 min

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The most common bacteria in CST IV include Gardnerella vaginalis , Megasphaera , Sneathia , and Prevotella species ( The composition of VMB is dynamic and continuously changes in reproductive-age women

The inclusion of this covariate did not meaningfully alter the significance of the main or interaction effects