These phosphorylation events correlate with enhanced integrin clustering and cytoskeletal reorganisation, observable through high-resolution confocal microscopy and atomic force microscopy measurements
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The cell lysate was clarified by centrifugation twice (10 minutes, 4C, 20,000 RPM) and 50 l was removed for derivatisation of thiols by addition of 1.5 l 1 M DTT, 1.5 l Mono-bromobimane and 45 l 1 M CHES pH 9
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