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(60) utilized next generation sequencing (NGS) and computational analysis to demonstrate that miR-23b-3p, acting as a tumor suppressor, can inhibit tumor Gln metabolism by targeting GLS1, thereby enhancing the sensitivity of liver tumor cells to sorafenib
Sometimes laboratory evaluation may be appropriate
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2.9 Assessment of apoptosis by flow cytometry Cells were seeded in cRPMI into 12-well plates at optimised seeding densities (HCT116: 400,000 cells/well, SW837: 500,000 cells/well) and allowed to adhere overnight at 37C in 5% CO 2 /95% humidified air
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