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After in vitro stimulation with CpG, B cells from older individuals produce significantly lower levels of IgM and IgA antibodies compared to younger individuals, highlighting a diminished capacity to respond to novel pathogens while maintaining responsiveness to frequently encountered antigens [357]

After blocking in 1% skim milk in Tw-PBS for 1 h at room temperature, the membrane was incubated with SMA (dilution 1:2000

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