The following flow antibodies were used: anti-mouse CD4 PE/Cy7 (clone GK1.5) (BioLegend, 100422), anti-mouse IL-17a PE (clone TC11-1810.1) (BioLegend, 506903), anti-mouse CD45 PerCP/Cy5.5 (clone 30-F11) (BioLegend,103131), anti-mouse GATA3 (BioLegend, 653809), antimouse/human CD11b PE/Cy7 (clone M1/70) (BioLegend, 101216), anti-mouse/human CD11b Brilliant Violet 605 (clone M1/70) (BioLegend, 101237), antimouse inducible NO synthase (iNOS) (Invitrogen, 125920), antimouse Foxp3 Pacific blue (clone MF-14) (BioLegend, 26410), antimouse/human Arg1 FITC (R&D Systems, IC5868F), anti-mouse F4/80 PerCP/Cy5.5 (clone BM8) (BioLegend, 123127), antimouse MGL2/CD301B (BioLegend, 146807), antimouse/human T-bet PE/Cy7 (clone 4B10) (Biolegend, 644824), antimouse/human RORt (APC, clone AFKJS-9) (Invitrogen, 17698882), antimouse Ly6G (clone 1A8) (BioLegend, 127613), and PE antimouse CD206 (clone C068C2) (BioLegend, 141705)
Your healthcare provider may increase your injection strength at each visit to compensate for the longer interval between doses
In doing so, fibrotic IVDs maintained the stability and biomechanics of the vertebra
AMPK activator AICAR was from Beyotime Biotechnology (Shanghai, China)
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