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Utilization of industrial waste from vegetable oils for riboflavin production by Candida guilliermondii DM 644

Apart from the low propensity of the GSAAT(C168S) and GSAAT(C190S) mutants to dimerize, these single substitution mutants not only failed to promote redox-dependent dimerization and oxidation of monomeric GSAAT, but even prevented it

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doi: 10.2144/fsoa-2020-0091 210 OrdonezA

The NAPstar3b variant was generated by site-directed mutagenesis on the pDONR207 NAPstar3 plasmid using the primer pair Pr1/Pr2 (Supplementary Table 6)