Lessons from (S)-6-(1-(6-(1-methyl-1H-pyrazol-4-yl)-[1,2,4]triazolo[4,3-b]pyridazin-3-yl)ethyl)quinoline (PF-04254644), an inhibitor of receptor tyrosine kinase c-Met with high protein kinase selectivity but broad phosphodiesterase family inhibition leading to myocardial degeneration in rats
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Most people tolerate it well, with only mild gastrointestinal side effects reported in some cases
In this complex, both, the FOXO4-derived region and the cationic cell permeability peptide contribute to the interaction
Culture medium consisted of Dulbeccos Modified Eagle Medium (DMEM): Hams F-12 Nutrient Mixture (1:1) supplemented with 100 U/mL Penicillin and 100 g/mL Streptomycin, 2 mM Glutamine, 0.5x N2 supplement, 2.6 ng/mL insulin, 2 nM dexamethasone, 20 ng/mL epidermal growth factor (EGF), and 20 ng/mL basic fibroblast growth factor (bFGF)