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For each gene of interest, two sgRNAs from our transporter-focused KO library were selected, and the corresponding forward and reverse oligos, flanked by BsmBI-cutting sites, were annealed and cloned into the modified lentiCRISPRv2 vector via Golden Gate assembly (Table 2)

The primary constituents of these bioactive compounds are betalains, accompanied by other components such as coumarins, carotenoids, sesquiterpenoids, triterpenes, and flavonoids (such as astragalin, tiliroside, rhamnocitrin, kaempferol, and rhamnetin), which possess antioxidant properties

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Abstract Glutathione is central to cellular redox chemistry

Optic Nerve Atrophy: Administering cyanocobalamin to patients with Leber's disease or hereditary optic nerve atrophy should be done cautiously, as it may worsen the condition