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The pre-stained cells were concentrated and immobilized on a 0.45 M, 47 mm diameter polycarbonate filter using a vacuum-filter system

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Mice and rats were anesthetized with subcutaneous ketamine/xylazine, the corneal surface was numbed with 0.5% proparacaine drops, and pupils were dilated with sequential drops of 0.5% tropicamide and 2.5% phenylephrine HCl

GIP was measured using ELISA as per the manufacturers instructions (Millipore)