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When you take care of yourself, you are taking better care of your baby

Determination of cell death by PI staining Cells were trypsinized, washed with PBS and suspended in 300 l Dulbeccos phosphate buffered saline (DPBS) supplemented with 0.2% BSA containing 0.3 g/ml propidium iodine (PI, Sigma-Aldrich)

Note: This is intended as a guide only

Although the procedure itself is straightforward, attention to detailsuch as using sterile supplies, selecting an appropriate diluent, adding BAC water slowly, minimizing unnecessary agitation, and storing the finished solution correctlycan help preserve peptide quality throughout the course of a study

These enzymes are involved in the metabolism of medium- and long-chain fatty acids (e.g