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To reconstitute a peptide vial: (1) Allow the vial to reach room temperature, (2) Sterilize the rubber stopper with an alcohol prep pad, (3) Draw the calculated amount of bacteriostatic water into a sterile syringe, (4) Slowly inject the bacteriostatic water down the inside wall of the vial to avoid foaming, (5) Gently roll the vial to dissolvenever shake, (6) Verify the solution is clear with no visible particles, and (7) Store refrigerated at 2-8C protected from light

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The culture period was 12 days, long enough to observe meaningful changes in follicle elongation and cell behavior