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Luciferase report gene assay Cellular cAMP determinations were based on an indirect method using a reporter plasmid containing the firefly luciferase gene under the control of multiple cAMP-responsive elements (CREs) and pRL-TK as a control vector (Promega Corp., Madison, WI), which has previously been used for determining D 1 R, D 2 R, and D 3 R signaling [31, 32]

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[DOI] [PMC free article] [PubMed] [Google Scholar] 230.Jeon O.H., Kim C., Laberge R.M., Demaria M., Rathod S., Vasserot A.P., Chung J.W., Kim D.H., Poon Y., David N., et al

Subsequently 500 pl of the sample was mixed with approximately 9pl of ThT stock solution in a separate sample container, to give a final ThT concentration of 20 pM

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