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Principle and Working Mechanism of Magnetic Bead-Based Protein Purification Magnetic beads contain a paramagnetic core encased in a polymer shell that can be functionalized for selective capture

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doi: 10.1016/j.foodchem.2020.128155, 128

Key features Simple and fast protocoluser-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period Broad range of kitsacross key analytes and targets within the most important metabolic pathways Consistentstrict quality control criteria to ensure reproducible and reliable results

Among them, the glutamate produced by the decomposition of glutamine via GLS not only provides the raw material for the synthesis of GSH but is also further metabolized into -ketoglutarate (-KG) in the mitochondria under the action of glutamate dehydrogenase (GLUD1) or transaminases and then enters the tricarboxylic acid cycle to produce ATP [29]