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Under these conditions, the skin was cut into small fragments of approximately 1 cm2, which were placed inside plate inserts with a 3 m pore membrane (# 10769-210

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While proliferation slows in ACLY KO HCC cells in the absence of acetate, the cells retain viability and continue to proliferate in the absence of exogenous acetate, in contrast to ACLY KO MEFs (Figure 1E

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This too is consistent with previous studies in which NAA was comparable between concussion and control participants at 72 h, 12 days 17 , 3 months 16 , and 1 year 17