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Briefly, cells were lysed on ice for 15 min in NETN lysis buffer (20 mM Tris pH 8.0, 100 mM NaCl, 0.5 mM EDTA, 0.5% NP40) supplemented with 10 g/mL aprotinin, 10 g/mL leupeptin, 10 g/mL pepstatin A, 1 mM sodium orthovanadate, 1 mM sodium fluoride, and 1 mM AEBSF (4-[two aminoethyl] benzenesulfonyl fluoride)

Proteins were visualized via chemiluminescence using the ECL Advance Western Blotting Detection Kit (Amersham Biosciences), and images were captured and analyzed by densitometry using a Kodak ID Version 3.6 Image Analyzer (Kodak, NY, United States)

That broader sense of things feeling calmer in the body less reactive, less unpredictable is something we hear consistently from clients at this stage

Timelines vary

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