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After one or two weeks pretreatment, a wounded area in the culture dish was made by scratching a straight line in the monolayer of fibroblasts using a sterile 1000-l pipette tip

Although the presence of NOX5 in human spermatozoa has not yet been confirmed, a recent publication in the mouse [46] employed Western blot analysis to record the presence of proteins exhibiting cross reactivity with antibodies against various components of the leukocyte NADPH oxidase complex (NOX2) including gp91phox, p67(phox), p47 phox and p40 (phox)

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doi: 10.1158/1541-7786.MCR-18-0748 31 LiottiACivita LaECennamoMCrocettoFFerroMGuadagnoEet al

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