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For the DDD -Ube2e2-COP1-c-Jun-STK40 complex, untagged DDB1 and DDA1, TSF-tagged human DET1 and STK40, N-terminal GST- and C-terminal His-tagged COP1, MBP-tagged Ube2e2 and c-Jun were co-transfected into Expi293F cells

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As a means of protecting the cell, there is genetic modulation