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Cells were cultured in Dulbeccos modified Eagles medium (DMEM) (Gibco, NY, USA) supplemented with 10 % foetal bovine serum (FBS) (Gibco, NY, USA) and were maintained in a humidified incubator at 37 C and 5 % CO 2

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We also need to take into account: The temperature at which the battery is being used The recommended maximum Depth of Discharge C ratings are commonly used on Lithium Ion batteries while Sealed Lead acid batteries tend to use the 20 hour rate