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All fibroblast cell lines were cultured in MEM (Life Sciences) supplemented with 15% FBS (Gemini Bio-Products) and 2 mM L-glutamine (Life Sciences) at 37 C with 5% CO 2
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We included peer-reviewed randomized controlled trials, meta-analyses, and high-quality narrative or systematic reviews written in English that evaluated the effects of GLP-1 receptor agonists (liraglutide, semaglutide, or related analogues), as well as dual GIP/GLP-1 receptor agonists (such as tirzepatide), exercise training, or their combination on weight loss, body composition, metabolic, or cardiovascular outcomes in adults with obesity or type 2 diabetes
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Furthermore, F 405 /F 488 is insensitive to other environmental factors, including potential non-specific interactions or reactions with intracellular proteins (Fig
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