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Gene transcription during CS is regulated at multiple layers such as the binding of transcription factors to cold-regulative elements located in the promoter [e.g

Cortellini A, Chiari R, and Ricciuti B, et al (2019) Correlations between the immune-related adverse events spectrum and efficacy of anti-PD1 immunotherapy in NSCLC patients Clin Lung Cancer 20 (4) 237 e1247 e1 PMID: 30885550 46

After washing with PBS, cells were lysed with lysis buffer containing 1% Triton X-100

Moreover, preclinical studies of individual peptides have not reported significant acute toxicity