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Apoptosis and necrosis of senescent chondrocytes After culturing with CuS and B2M-CuS NPs (50 g mL 1 ) for 24 h, senescent ATDC5 cells were collected and incubated with 100 L of Annexin V-FITC and PI solution (BD, USA) for 20 min at RT

Weight management through lifestyle modification for the prevention and management of type 2 diabetes: rationale and strategies: a statement of the American Diabetes Association, the North American Association for the Study of Obesity, and the American Society for Clinical Nutrition