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These limitations highlight the need for systematic temporal studies to define the optimal stages of hepatic differentiation and functionality in iHLOs

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As class B1 peptide agonists engage their receptors via a two-domain interaction, their efficacy for G protein mediated signalling is influenced by multiple factors, including the nature of interactions with the ECD and TMD, contributing to both peptide affinity and how ligand-receptor interactions influence G protein binding, nucleotide exchange and G protein dissociation from the receptor