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GHK exists naturally in saliva and urine as well, released from extracellular matrix proteins including SPARC and the collagen 2(I) chain during tissue injury, functioning as a matrikine damage signal that activates repair mechanisms

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Also stress , be it physical or mental can deplete B12

Retention Efficiency: Ex-vivo wash-off studies showed that 75% of the peptide remained on the scalp substrate after rinsing, compared to only 15% for the free solution