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Lyophilization (freeze-drying) removes water from the peptide matrix, eliminating the solvent-mediated motion that drives thermal unfolding

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Therefore, morphology of Lyz + secretory granules is used as a functional marker for Paneth cell phenotype classification, and both inflammation and epithelial mitochondrial dysfunction have previously been shown to correlated with reduced Paneth cell granularity (8, 9)

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