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For those patients, the experience can look very different

IMAC is a method to purify a protein with an affinity for metal ions, such as Co 2+ , Ni 2+ , Cu 2+ , or Zn 2+ , immobilized on a matrix (Yang et al ., 1998)

Acknowledgments The authors are grateful to Claudia Jonak (Vienna, Austria) for kindly providing seeds of the g6pd5-1 g6pd6-2 double mutant, and thank Paulina Heinkow for sample preparation and technical assistance within the MS Proteomics Unit Biology of Plants of the University of Mnster

The same cells were imaged with the 60 oil immersion objective for GSH concentration and the 60 water immersion objective for the redox potential

Monitoring results, tweaking doses, and troubleshooting issues requires consistent attention over protocol duration