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The synergistic effects of these microbial shifts, further potentiated by the anti-inflammatory properties of omega-3 fatty acids, suggest that the Mediterranean diet fosters a holistic anti-inflammatory microbial environment (Nikdasti et al., 2025)

Hydrogen peroxide solution (H 2 O 2 3%) was added to the slides to prevent endogenous peroxidase activity, followed by primary antibodies (anti-BAX 1:100) (ABclonal , USA) and (anti-GFAP 1:100) (Diagnostic BioSystems, USA) to be incubated with the slides overnight

BPC-157's primary proposed mechanisms VEGF/angiogenesis and gastric/gut-lining signalling are directly relevant to both vascular and gastrointestinal research contexts

Flow cytometry was performed on a LSRFortessa X-20 (Becton Dickinson, Franklin Lakes, NJ) and data were analyzed using FlowJo (v7.1.6, TreeStar)