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The reconstitution-related concern with these formats is not about mixing it is about whether the molecule reaches systemic circulation at all

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Late-life targeting of the IGF-1 receptor improves healthspan and lifespan in female mice

Pellets were then resuspended in 1 mL ice-cold lysis buffer (50 mM Tris pH 8.4, 30 mM threonine, 0.1 mM DTPA) and lysed by sonication, still inside the anaerobic chamber

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