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(2018) 195:11520.e3
Depending on the wavelength, the energy may or may not produce heat
Cell cultures Cortical neurons in primary culture were prepared from fetal Wistar rats (E16), seeded at 2.510 5 cells per cm 2 in 6- or 12-well plates previously coated with poly-D-lysine (15 g ml 1 ) in DMEM (Sigma, Madrid, Spain) supplemented with 10% (v/v) fetal calf serum (Roche Diagnostics, Heidelberg, Germany)
doi: 10.3389/fpsyt.2019.00892 31 GuoJHeCSongHGaoHYaoSDongSSet al
Five sets of experiments on cell cultures were performed: with 5 mM glucose (NG), NG + 100 nM celastrol, 30 mM D-glucose (HG), HG + 100 nM celastrol, and HG + 1 M Fer-1
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