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Johnson aims for a 10 percent deficit based on research that has shown calorie deficits increase longevity, though we should note that almost all calorie restriction longevity research has been conducted with animal subjects, and scientists still arent sure why the effect takes place (6)

Post-prandial oxidative stress in urine The oxidative stress in urinary (Isoprostane molecules) were marked elevated in all the meal challenges (except for HC meals), in both the MHL subgroup (0.37 to 0.75, 0.57 to 0.76) and MUO subgroup (0.37 to 0.47, 0.43 to 0.46)

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MC4R: Located in the central nervous system, particularly the hypothalamus, influences sexual behavior, erectile function, and energy homeostasis