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AAV was then purified through iodixanol gradient ultracentrifugation (for 2 h 40 min at ~381,600 g [rotor: 70Ti, Beckman Coulter] at 14C) and titered by qPCR

The assay served as a surrogate measure for assessing tissue swelling, as intervertebral disc has high content of GAG, it is known that structural changes result in degeneration of disc which is preceded and accompanied by loss of GAGs

One hour after the oral administration of 32 P-dichlorvos, maximum concentrations of radioactivity are found in the kidneys, liver, stomach, and intestines

abnormalities in thermoregulation, cardiovascular function, and tissue perfusion are important factors leading to multi-organ injury

In our practice we notice that the Dutch College of General Practitioners (NHG) guidelines[1] are not sufficiently effective for a lot of patients, and that symptoms appear again after switching to injections once every two or three months