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PMID: 30575050

Step-by-Step Reconstitution 01 Bring vial to room temperature Let the lyophilized NAD+ vial sit at room temperature for 5-10 minutes before reconstitution

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It is reasonable to assume that changes in action potential shape could affect calcium influx into presynaptic boutons and hence the probability of vesicle release, suggesting that restoration of action potential shape may also influence release properties