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Tirzepatide additionally activates GIP receptorsa secondary appetite and glucose control pathway that semaglutide alone does not engage

The formaldehyde-containing DMEM was removed and cells were washed 3 times with 20ml of ice-cold PBS supplemented with 1x complete protease inhibitor cocktail

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A high C-rated pack might tolerate that better than a low C-rated pack, but it still accelerates wear compared to slow charging

The cells were then washed three times with PBS and incubated with Oil Red O working solution (G1260, Solarbio) for 30 min, protected from light